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KMID : 0613820180280020176
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Journal of Life Science
2018 Volume.28 No. 2 p.176 ~ p.186
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Anti-proliferating Effects and Gene Expression Profiles through Antioxidant Activity of Porphyra yezoensis Fractions on Human HepG2 Cell Lines
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Oh Youn-Jeong
Kim Jung-Min
Bang In-Soek
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Abstract
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In this study, the total polyphenol contents, antioxidant activities and anti-proliferation effects of HepG2 cell lines in organic slovent fractions obtained from the main methanolic extract of P. yezoensis were analyzed. The polyphenol content of the CHCl3 fraction was 10.3 μg/mg, slightly less than 13.08 μg/mg of the water fraction, but ED50 estimated by measuring DPPH free radical scavenging activity exhibited the highest 16.96 μg/ml in the CHCl3 fraction. The proliferation effects of CHCl3 and EtOAc fraction toward HepG2 cells inhibited in a dose-dependent manner, showed 90% inhibition when treated for 24 hr at 900 μg/ml of CHCl3 fraction. Meanwhile gene expression patterns in HepG2 cells treated 50 μg/ml of CHCl3 fraction were identified with microarray analysis. Concerning the efficacy of P. yezoensis, gene ontology analysis explored the genes associated with response to molecule of bacterial origin, vitamin D metabolic process, and response to nutrient. Thus IL6R, CYP1A1 were selected as significant genes based on expression patterns of HepG2 cells, and pathway analysis indicates that ARNT might be considered as a upstream regulator. Also, expression analysis of IL6R and CYP1A1, activity of upstream regulator ARNT in HepG2 cells was confirmed based on Western blotting analysis at the protein level after being treated with 50 and 100 μg/ml of CHCl3 fraction.
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KEYWORD
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Antioxidant activity, gene ontology, HepG2, microarray, P. yezoensis
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